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Super-Resolution Microscopes Capture Live Cell Structure Images
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Microscopes
Scientists and professors at the Department of Pharmaceutical Chemistry and Department of Biochemistry and Biophysics at the University of California San Francisco and at the Georgia Institute of Technology are developing a technique that has the technology to offer researchers access to a “super-resolution microscope to perform live-cell imaging.” This image allows scientists to identify single molecules.
According to the researchers, the “discovery of super-resolution fluorescence microscopy techniques such as photoactivated localization microscopy (PALM) and stochastic optical reconstruction microscopy (STORM) has overcome the diffraction limit of light microscopes.” The importance of this technology is that it allows scientists to capture and record the “emission of light from a sample’s single molecule.”
This is a significant breakthrough as it removes the “assumption or estimation factor” in determining the number of molecules in an image. The compressed sensing technology works with higher density molecules than in the past and this allows lab technologists to better understand the life cycle of a cell and may provide more detailed insight into biological questions. The technique provides “unprecedented temporal resolution and required spatial resolution to take images of dynamic single cellular structures.”
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Detailed results of this study were first published in Nature Methods Journal.